Fig. 2

Vibrio alginolyticus virulence genes (collagenase, VptoxR, tdh) using Multiplex PCR. Agarose gel electrophoresis (1.5% agarose) of the collagenase (lanes 1–4) amplification products (738 bp) of V. alginolyticus strains. Lane: M, molecular weight marker. Neither amplification products (296 bp) for VptoxR, nor amplification products (270 bp) for tdh. Lanes 1, 2, 3, and 4 refer to VAK4, VASk3, VAM6, and VASp1 isolates, respectively