Molecular and phenotypic identification of bacterial species isolated from cows with mastitis from three regions of Poland

Table 1 List of primers used in the study and predicted sizes of PCR products for the amplification of E. coli, S. aureus, S. agalactiae and S. uberis

Studied species	Name	Primers	Source	Product size (bp)
Escherichia coli	EC_F	F: GGTAACGTTTCTACCGCAGAGTTG	[66]	460
Escherichia coli	EC_R	R: CAGGGTTGGTACACTGTCATTACG	[66]	460
Staphylococcus aureus	SA_F	F: AATCTTTGTCGGTACACGATATTCTTCACG	[85]	100
Staphylococcus aureus	SA_R	R: CGTAATGAGATTTCAGTAGATAATACAACA	[85]	100
Streptococcus agalactiae	GBS_F	F: CGATTCTCTCAGCTTTGTTA	[86]	780
Streptococcus agalactiae	GBS_R	R: AAGAAATCTCTTGTGCGGAT	[86]	780
Streptococcus uberis	GBS_F	F: TCGCGGTATTGAAAAAGCAACAT	[66]	400
Streptococcus uberis	GBS_R	R: TGCAATAATGAGAAGGGGACGAC	[66]	400

ISSN: 1746-6148