Table 1 Bacterial strains and plasmids used in this study
Bacterial strains/Plasmids | Descriptiona | Reference |
|---|---|---|
Strains | Â | Â |
CZ130302 | Serotype Chz S. suis strain | Collected in our lab |
ΔzmpC | Deletion mutant of zmpC with CZ130302 background | This study |
ΔzmpE | Deletion mutant of zmpE with CZ130302 background | This study |
ΔzmpN | Deletion mutant of zmpN with CZ130302 background | This study |
ΔzmpB | Deletion mutant of zmpB with CZ130302 background | This study |
ZY05719 | Serotype 2 S. suis strain | Collected in our lab |
DH5α | Cloning host for maintaining the recombinant plasmids | Invitrogen, USA |
BL21 (DE3) | Host for expressing proteins | Invitrogen, USA |
Plasmids | Â | Â |
Pet28a(+) | Expression vector, Kanr | Invitrogen, USA |
Pet28a-ZmpC-M26 | Pet-28a containing zmpC-M26 gene, Kanr | This study |
Pet28a-ZmpE-M26 | Pet-28a containing zmpE-M26 gene, Kanr | This study |