Fig. 4

Histopathological findings of gills. (a) Inflammatory cells infiltration (arrowhead) and presence of numerus cysts of Acanthamoeba sp. (arrows) inside the hyperplastic epithelial tissue of gills. (b) Higher magnification shows cysts with double-layered walls and central nucleus (arrows). Inflammatory cells infiltration (arrowhead). (c) Gill filament characterized by spongiosis (SP) of hyperplastic (H) epithelium. Cysts of Acanthamoeba sp. (arrowheads) (H&E). (d) The Masson’s trichrome staining highlights the cysts of Acanthamoeba sp. (arrowheads)