Fig. 1

Kaplan-Meier survival curve showing higher incidence of new intramammary infections by major mastitis pathogens in udder quarters in dairy cows during the first month of lactation with high expression (geometric mean fluorescence intensity) of CTLA-4 at parturition (A; P = 0.001) and PD-1 at 14 days before the expected day of calving (B; P = 0.03) by blood T-lymphocytes. CTLA-4: cytotoxic T-lymphocyte-associated antigen-4; PD-1: programmed cell death protein 1. A drop in survival probability indicates the occurrence of new IMIs (“failure”) by major mastitis pathogens within that specific period