Fig. 2

CLSM analysis of baicalin treated A.lwoffii pre-biofilms. A.Lwoffii biofilm was formed for 24 h at 37 °C on chambered coverglass slides. After washing, biofilms were treated with baicalin for 3 h at 37 °C as described and subsequently stained with SYTO 9 and PI for 20 min in the dark. Images were acquired by CLSM. Using BiofilmQ software for image processing.. A and B 3D and orthogonal views biofilm representation in the objective of 20X in the control group. C and D 3D and orthogonal views biofilm representation of baicalin in the objective of 20X. E total fluorescence intensity of biofilms (PI + SYTO9). F represents the number of biofilms. G bottom area of biofilms. H represents the volume of biofilms. I represents the surface area of biofilms. Unpaired t-test (two-tailed) was used to measure statistical significance. * P < 0.05, ** P < 0.01 compared with the control group