Fig. 3

Analysis of differential gene expression. The preformed biofilm was treated with 4 MIC baicalin for 3 h, and the upper culture medium in cell culture flasks was collected and centrifuged. The supernatant was taken and frozen in liquid nitrogen for storage. There were 6 samples in both the baicalin group and the control group. The samples were analyzed by LC–MS, and the data were extracted and preprocessed using Mas-terView (SCIEX). A Volcano plots showed the fold change of differential gene in baicalin group vs control group. The green dots represent significantly down-regulated genes (466), the red dots represent significantly up-regulated genes (367), and grey dots represent non-significantly changed differential genes. B Venn diagram. C Cluster analysis diagram of differentially expressed genes, the twenty-four hours A.Lwoffii pre-biofilms was treated with baicalin (4 MIC) for 3h, and the untreated biofilms was used as the control. All experiments were performed at least in triplicate, and there were 6 samples in both the baicalin group and the control group. D GO annotation enrichmentan analysis diagram and (E) KEGG enrichment analysis diagram. The p value is the significance of enrichment of this metabolic pathway. The ordinate is the name of the metabolic pathway, the abscissa is the rich factor. The larger the rich factor, the more metabolites enriched in the pathway. The size of the dots represents the number of differential metabolites enriched into the pathway