Fig. 4

Global gene expression in canine mammary gland tumor cell lines. (A) Hierarchical clustering of MGT cell lines, MDCK and TP53 K/O fibroblast. (B) Heatmap plot of differentially expressed genes from the comparison of RNA-seq from Canine tumor cells with normal cells filtered by the fold change and p-value (< 0.05). High expression is indicated in red and low expression is indicated in blue. (C) Volcano plot of upregulated (n = 296), and downregulated (n = 212) genes, p-value < 0.05. (D) Bar chart for KEGG pathway enrichment analysis of upregulated genes in canine tumor samples. (E) Bar chart for KEGG pathway enrichment analysis of downregulated genes in canine tumor samples