Fig. 5
From: Role of LsrR in the regulation of biofilm formation in mammary pathogenic Escherichia coli

The binding ability of LsrR to the fimA promoter was determined by gel shift assays. Increasing LsrR amounts were incubated with probes lsrR and fimA promoters (p- lsrR and p- fimA). In each panel, from lanes (1) to (4), the LsrR concentrations were 0, 0.5, 1, and 2 µmol, respectively; the amounts of probes in all lanes were 100 ng. (A) Positive control group, the binding ability of LsrR and lsrR promoter; (B) The binding ability of LsrR and fim promoter