Fig. 1

Amino acid sequences of Acrossocheilus fasciatus LEAP2 and its homologues based on multiple alignments. A 70% threshold was employed for shading, with grey indicating similar residues, black indicating identical residues, and ‘-’ indicating alignment gaps. The predicted cleavage site for the signal peptide or mature peptide is designated with ‘↓’, and the four conserved cysteine residues in the mature peptide are denoted by ‘*’. Accession numbers of the sequences used are listed in the Fig. 2 legend