Fig. 2
Phylogenetic (Neighbor-joining) analysis of complete amino acid sequences of LEAP2 using the MEGA X program. The values at the forks indicate the percentage of trees in which this grouping occurred after bootstrapping (1000 replicates; shown only when > 60%). The scale bar shows the number of substitutions per base. Accession numbers of sequences used are as follows: Ctenopharyngodon idella LEAP2 (FJ390414); Ctenopharyngodon idella LEAP2B (KT625603); Megalobrama amblycephala LEAP2 (JQ344324); Cyprinus carpio LEAP2A (KC551971); Cyprinus carpio LEAP2B (KC551972); Oncorhynchus mykiss LEAP2A (NM_001124464); Oncorhynchus mykiss LEAP2B (NM_001124465); Oncorhynchus mykiss LEAP2C (GQ870279); Pundamilia nyererei LEAP2 (XM_005750928); Larimichthys crocea LEAP2A (KJ024787); Larimichthys crocea LEAP2C (KJ024789); Plecoglossus altivelis LEAP2 (KJ412462); Paralichthys olivaceus LEAP2 (EU586111); Oryzias latipes LEAP2A (XM_004079958); Oryzias latipes LEAP2C (XM_004074820); Xiphophorus maculatus LEAP2A (XM_005806413); Xiphophorus maculatus LEAP2C (XM_005810019); Ictalurus furcatus LEAP2 (AY845142); Ictalurus punctatus LEAP2 (AY845141); Takifugu rubripes LEAP2C (XM_011604959); Danio rerio LEAP2A (BC162807); Danio rerio LEAP2C (XM_009295063); Salmo salar LEAP2B (XM_014129528); Salmo salar LEAP2C (GQ870280); Oreochromis niloticus LEAP2A (XM_003457723); Oreochromis niloticus LEAP2C (XM_013270789); Anguilla japonica LEAP2 (KP893812); Cynoglossus semilaevis LEAP2A (XM_008320540); Cynoglossus semilaevis LEAP2A (XM_008324342); Miichthys miiuy LEAP2 (KJ000088); Mus musculus LEAP2 (BC089593); and Homo sapiens LEAP2 (BC070199)