Overview on the role of dietary Spirulina platensis on immune responses against Edwardsiellosis among Oreochromis niloticus fish farms

Table 1 Primers sequences, target genes, amplicon sizes and cycling conditions

Target gene	Function	Sequences	Size (bp)	Annealing ˚C/sec	Reference
*gyrB1*	ATPase domain	F: GCATGGAGACCTTCAGCAAT R: GCGGAGATTTTGCTCTTCTT	415	50/40	[35]
*Cds1*	Chondroitinase	F: TCTCCACCCATAATGCCACG R: CAAACGGCGTCGTGTAGTCG	435	55/40	[36]
*edwI*	AHL-synthase	F: ATCCGCAGCATCGAATGGCT R: GAAGGATAACGATGTGGTGT	360	55/40
*qseC*	sensor protein	F: CAGCAGTAGCAGGATCACCA R: ATGGACGTATGCTGCTCAAC	260	55/40
*pvsA*	vibrioferrin synthesis	F: CTGGAGCAGTACCTCGACGG R: CGATGCTGCGGTAGTTGATC	313	55/40
*β-actin*	House keeping	F: AGCAAGCAGGAGTACGATGAG R: TGTGTGGTGTGTGGTTGTTTTG	135	58.5/30	XM_003443127.5
*IL-1β*	Pro-inflammatory	F: T GCTGAGCACAGAATTCCAG R: GCTGTGGAGAAGAACCAAGC	172	60/30	XM_019365841.2
*TNF-α*	Pro-inflammatory	F: CCAGAAGCACTAAAGGCGAAGA R: CCTTGGCTTTGCTGCTGATC	82	59.9/30	AY428948.1

ISSN: 1746-6148