Preliminary investigation of potential links between pigmentation variants and opioid analgesic effectiveness in horses during cerebrospinal fluid centesis

Table 6 Haplotype analysis of significant SNPs within the Agouti signalling protein gene

Haplotype^a	Frequency	diff	P-value ^Ƹ
Original model
TTGTCC	0.489	2.26	-
TTATCC	0.267	0.34	0.040*
TTGATA	0.200	0.42	0.035*
Rare	0.045	-0.80	0.035*
MC1R^bcovariate model
TTGTCC	0.491	1.93
TTATCC	0.265	0.39	0.016*
TTGATA	0.198	0.32	0.141
	0.046	- 0.51	0.208
MC1RC/T		0.39	0.095
MC1RC/C		0.76	0.008*
All covariate^cincluded model
TTGTCC	0.500	1.79	-
TTATCC	0.256	0.58	0.013*
TTGATA	0.198	1.08	0.004**
Rare	0.046	0.02	0.962
MC1R C/T	-	0.33	0.139
MC1R T/T	-	0.16	0.620
DR Hyd^d	-	-22.02	0.316
Male	-	-0.67	0.061
Thoroughbred	-	0.16	0.641
Warmblood	-	0.87	0.047*
Age	-	0.02	0.491

^ƸGeneralised linear model
*Passing nominal significance threshold (P<0.05)
**Passing Bonferroni significance threshold (P<0.0045
^aIncluded locations in order of inclusion: Chr22:g.26009603, Chr22:g.26010152, Chr22:g.26010317, Chr22:g.26010659, Chr22:g.26010673, & Chr22:g.26010738.
^bInclusion of Chr3; g.36979560 as covariate indicating the MC1R mutation
^cCovariate included model with MC1R genotype, age, breed, sex and hydromorphone dosage rate
^dDosage rate of Hydromorphone (mg/kg BW)

ISSN: 1746-6148