Table 2 The primers and probe used for the detection of the 16 S rRNA Rickettsia gene using Taqman Real-time PCR assay
From: Diversity of Rickettsia species in collected ticks from Southeast Iran
Primer name | 5′-primer sequences-3′ | Target locus | Amplicon, bp | Reference |
|---|---|---|---|---|
Forward | 5’-CGCAACCCTYATTCTTATTG-3’ | 16 S rRNA | 149 | [12] |
Reverse | 5’-CCTCTGTAAACACCATTGTAGCA-3’ | |||
probe | 6-FAM-TAAGAAAACTGCCGGTGATAAGCCGGAG-TAMRA |