Fig. 12

An immunohistochemical staining of anti-VEGF in the intestines of control and infected fish showing the significant difference in the number of mast cells between the control and infected fish. (A-C) The intestine of control fish shows minimal immunoreactivity of VEGF (arrowheads), in figure C, specifically the interaction in mast cells between smooth muscle fibers (SMF) and the mild reactivity of the endothelium (En). (D-F) The intestine of infected fish shows numerous mast cells (arrowheads). The degranulation of mast cells is observed (double arrowheads), in figure F, the response of mast cells is observed, particularly the significant increase in the size of mast cells between smooth muscle fibers (SMF) and extensive responsiveness in endothelium compared to the control