Table 2 Identity, sources, and working dilution of antibodies used in the present immunohistochemical analysis
Primary antibody | Supplier | Origin | Dilution | Incubation | Antigen retrieval | Biotinylated secondary antibody |
|---|---|---|---|---|---|---|
CD68 (Macrophage Marker) Ab-3 (Clone KP1) | Mouse Anti-CD68 (Thermo Fisher Scientific Lab Vision Corporation, Fremont, USA) | Mouse Monoclonal Antibody Cat. #MS-397-R7 | 1:100 | Over night | boiling in citrate buffer (pH 6.0), 20 min | Goat anti-Mouse IgG (H + L) Secondary Antibody (Catalog # 31,569) Dilution ; 1:100 One hour at room temperature |
VEGF | Rabbit anti -VEGF (Thermo Fisher Scientific Waltham, MA, USA)) | Rabbit VEGF Polyclonal Antibody (clone: RB-222- P0) (Cat.no PA1- 21,796) | 1:100 | Over night | boiling in citrate buffer (pH 6.0), 20 min | Goat anti-rabbit secondary antibody (Cat. no. K4003, EN Vision + TM System Horseradish Peroxidase Labelled Polymer; Dako). Ready to use (30 min at room temperature) |
PCNA | Mouse Recombinant Monoclonal PCNA antibody (Abcam) | Monoclonal antibody (Cat.no ab264494) | 1:200 | Over night | boiling in citrate buffer (pH 6.0), 20 min | Goat anti-rabbit secondary antibody (Cat. no. K4003, EN Vision + TM System Horseradish Peroxidase Labelled Polymer; Dako). Ready to use (30 min at room temperature) |