Table 1 Components of the Fixative

Bouin’s solution

Picric acid saturated aqueous solution

750 mL

40% formaldehyde

250 mL

Glacial acetic acid

50 mL

Karnovsky fixative

Paraformaldehyde, 25% freshly prepared

10 mL

Glutaraldehyde 50%

10 mL

Na-phosphate buffer (0.1 M, pH 7.4)

50 mL

Distilled water

30 mL

Na-phosphate buffer (0.1 M, pH 7.4)

Solution A

Na₂HPO₄·2H₂O

17.02 g

Distilled water

600 mL

Solution B

NaH₂PO₄·H₂

6 g

Distilled water

200 mL

Using solution

Solution A

580 mL

Solution B

219 mL

Citrate buffer (pH 6.0)

Solution A

Citrate C₆H₈O₇·H₂O

21 g

Distilled water

1 L

Solution B

Sodium citrate Na₃C₆H₅O₇·2H₂O

29.41 g

Distilled water

1 L

Using solution

Solution A

9 mL

Solution B

41 mL

Distilled water

Add 500 mL