Table 2 Identity, sources, and working dilution of antibodies used in the present immunohistochemical analysis
Primary antibody | Supplier | Origin | Dilution | Incubation | Antigen retrieval | Biotinylated secondary antibody |
|---|---|---|---|---|---|---|
CD68 (Macrophage Marker) Ab-3 (Clone KP1) | Mouse Anti-CD68 (Thermo Fisher Scientific Lab Vision Corporation, Fremont, USA) | Mouse Monoclonal Antibody Cat. #MS-397-R7 | 1:100 | Over night | boiling in citrate buffer (pH 6.0), 20 min | Goat anti-rabbit secondary antibody (Cat. no. K4003, EN Vision + TM System Horseradish Peroxidase Labelled Polymer; Dako). Ready to use (30 min at room temperature) |
Anti-CD3 antibody [CD3-12] | (ab11089), Abcam |  | 1:100 | Over night | boiling in citrate buffer (pH 6.0), 20 min | Goat anti-rabbit secondary antibody (Cat. no. K4003, EN Vision + TM System Horseradish Peroxidase Labelled Polymer; Dako). Ready to use (30 min at room temperature) |
Ant i- CD21 antibody | (Abcam) | Rabbit monoclonal [SP186](ab227662) | 1:100 | Over night | boiling in citrate buffer (pH 6.0), 20 min | Goat anti-rabbit secondary antibody (Cat. no. K4003, EN Vision + TM System Horseradish Peroxidase Labelled Polymer; Dako). Ready to use (30 min at room temperature) |