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Fig. 2 | BMC Veterinary Research

Fig. 2

From: Development of polymerase chain reaction-lateral flow dipstick assay for detection of Mycoplasma bovis in cattle

Fig. 2

The Primer and PCR-LFD strips specificity verification. (A) Determination of the analytical specificity of oppD by real-time qPCR. (B) Analytical specificity of the PCR assay. (C) Cross‑reactivity of the PCR-LFD strips. Only the M. bovis was amplified and there were no cross reactions with other pathogens tested. Line 1, M. bovis; line 2, M. bovis Xinjiang isolate; line 3, Mycoplasma ovine pneumonia; line 4, Escherichia coli; line 5, Pasteurella multocida; line 6, Brucella abortus; line 7, Bovineviral diarrhea virus; line 8, Mycobacterium bovis

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