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Table 1 Camparision of polymerase chain reaction-lateral flow dipstick assay and real-time qPCR assay on clinical samples

From: Development of polymerase chain reaction-lateral flow dipstick assay for detection of Mycoplasma bovis in cattle

PCR-LFD

Real-time qPCR

Positive

Negative

Total

Sensitivity (%)

Specificity (%)

Accuracy (%)

Positive rate (%)

Positive

42

0

42

97.67

   

Negative

1

154

155

 

100

  

Total

43

154

197

  

99.49

21.82 (43/197)

Positive rate (%)

21.32 (42/197)

      
  1. Both positive = 42, both negative = 154, number of samples = 197. The following equations were used: sensitivity (%) = (both positive/positive in real-time qPCR) × 100%; specificity (%) = (both negative/negative in real-time qPCR) × 100%; accuracy (%) = (both positive + both negative)/total) × 100%
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BMC Veterinary Research

ISSN: 1746-6148

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