Fig. 7
From: Development and validation of a droplet digital PCR assay for Nipah virus quantitation
Comparison of RT-ddPCR and qPCR for the detection of simulated NiV positive samples. Serum, tissue and feed samples were used to prepare 45 simulated positive samples and 30 NiV-free negative samples. The NiV armored RNA virus (1.19 × 105 to 1.19 × 101 copies/reaction) was separately spiked into three matrices, namely NiV-free serums, tissues, and feeds, to simulate samples of various concentrations. Analysis results of 75 simulated actual samples by ddPCR (A), qPCR (B) and differential analysis via ddPCR (C). Each sample was repeated 3 times