Table 2 Analytical sensitivity of NiV RT-ddPCR
From: Development and validation of a droplet digital PCR assay for Nipah virus quantitation
NiV armored RNA virus concentration (copies/reaction) | RT-ddPCR | qPCR | ||||
|---|---|---|---|---|---|---|
Mean values (copies/reaction) ± SD | Positive/ Reactions | RSD | Mean Ct values ± SD | Positive/ Reactions | RSD | |
1 × 103 | 1077.20 ± 44.44 | 30/30 | 0.56% | 29.56 ± 0.07 | 30/30 | 0.25% |
1 × 102 | 121.78 ± 13.23 | 30/30 | 2.20% | 32.63 ± 0.26 | 30/30 | 0.78% |
1 × 101 | 10.81 ± 1.20 | 30/30 | 4.60% | 32.20 ± 10.89 | 27/30 | 33.83% |
7.5 | 7.43 ± 0.85 | 30/30 | 11.38% | 29.47 ± 14.83 | 17/30 | 50.30% |
5 | 5.02 ± 1.63 | 27/30 | 32.47% | 22.46 ± 18.37 | 25/30 | 81.78% |
2.5 | 2.06 ± 1.48 | 21/30 | 71.71% | 18.10 ± 19.36 | 21/30 | 106.96% |
1 | 0.99 ± 1.20 | 17/30 | 120.47% | / | 0/30 | / |