Fig. 2

Characterization of the isolated cBM-MSCs. (A) Morphology of cBM-MSCs. (B) RT-qPCR analysis of the expression of stemness (REX1 and OCT4) and proliferation (KI67) marker of cBM-MSCs. (C) Flow cytometry analysis of the MSCs surface markers. (D) Alizarin red s, (E) Alcian blue, and (F) Oil red O staining for assessment of multilineage differentiation potential of cBM-MSCs through osteogenic, chodrogenic, and adipogenic lineage in vitro