Molecular and in silico analyses for detection of Shiga toxin-producing Escherichia coli (STEC) and highly pathogenic enterohemorrhagic Escherichia coli (EHEC) using genetic markers located on plasmid, O Island 57 and O Island 71

Table 1 Primers used for identification of virulence/genetic markers and serogroups of studied STEC/EHEC isolates

Primer name	Sequence (5’-3’ direction)	Amplicon size (bp)	Reference
Virulence markers
stx1	ATAAATCGCCATTCGTTGACTAC	180	[22]
	AGAACGCCCACTGAGATCATC
stx2	GGCACTGTCTCTCTGAAACTGCTCC	255
	TCGCCAGTTATCTGACATTCTG
stx2f	AGATTGGGCGTCATTCACTGGTTG	428	[23]
	TACTTTAATGGCCGCCCTGTCTCC
eae	GACCCGGCACAAGCATAAGC	384	[22]
	CCACCTGCAGCAACAAGAGG
ehxA	GCATCATCAAGCGTACGTTCC	534
	AATGAGCCAAGCTGGTTAAGCT
ecf1	TATCAGCACCAAAGAGCGGGAACA	99	[30]
	CCCTTATGAAGAGCCAGTACTGAA
Z2098	ACATCACAGGCTTCCTGAGC	423	[31]
	GGAACGTGCCTCCGAGATAG
Z2099	TTCAACAGTAGCGCAGGCAA	266
	CAAGCAGGGGCGGTTACTTT
nleA	ATGAACATTCAACCGACCATACAATCTG	1326	[32]
	TTAGACTCTTGTTTCTTGGATTATATCA
*Serogroups*
O26	CAATGGGCGGAAATTTTAGA	155	[26]
	ATAATTTTCTCTGCCGTCGC
O45	TGCAGTAACCTGCACGGGCG	238
	AGCAGGCACAACAGCCACTACT
O55	TCCTTATTTGTGTCGGGGG	207	[27]
	CCAGGAAAGCTGCCAATTATC
O80	TGAGAGCCAAGATCCAAGCA	158	[29]
	TGGGCCATATTCGAAGTTTGAA
O91	TTGCATCTGGCGCAATAAACACGG	616	[26]
	ACACCATCCCAAATACCTGCTTGC
O103	TTGGAGCGTTAACTGGACCT	321
	GCTCCCGAGCACGTATAAAG
O104	TGAACTGATTTTTAGGATGG	351
	AGAACCTCACTCAAATTATG
O111	TGTTTCTTCGATGTTGCGAG	438
	GCAAGGGACATAAGAAGCCA
O113	TGCCATAATTCAGAGGGTGAC	514
	AACAAAGCTAATTGTGGCCG
O121	TCCAACAATTGGTCGTGAAA	628
	AGAAAGTGTGAAATGCCCGT
O128	ATGATTTCTTACGGAGTGC	782
	CTCTAACCTAATCCCTCCC
O145	TTCATTGTTTTGCTTGCTCG	750
	GGCAAGCTTTGGAAATGAAA
O146	ATTCGGGTAACGACCCTGTGTTGA	378	[28]
	AGACTGCTAATGCAAGGAACATGG
O157	TCGAGGTACCTGAATCTTTCCTTCTGT	894	[26]
	ACCAGTCTTGGTGCTGCTCTGACA

ISSN: 1746-6148