Fig. 1

Establishment of the reaction system and the results of the optimization: (A) Results of establishing the basic reaction system. (a) SS serotype 2 genomic DNA, (b) SS serotype 14 genomic DNA, (c) and (d) Negative control. (B) Optimization results of probe concentration for the EA-probe qPCR Method. Probe concentration: (a) 0 µM, (b) 0.1 µM, (c) 0.2 µM, (d) 0.3 µM, (e) 0.4 µM, (f) 0.5 µM, (g) 0.6 µM, (h) 0.7 µM. (C) Optimization results of RNase H2 enzyme concentration for EA-probe qPCR Method. RNase H2 enzyme dosage: (a) 0 mU, (b) 5 mU, (c) 10 mU, (d) 15 mU, (e) 20 mU, (f) 25 mU, (g) 30 mU, (h) 35 mU