Fig. 4

Principle of EA-probe qPCR Method. The scissors represent the hydrolytic activity of RNase H2. The left side of the R nucleotide contains the quencher dye, while the right side contains the reporter dye.
Principle of EA-probe qPCR Method. The scissors represent the hydrolytic activity of RNase H2. The left side of the R nucleotide contains the quencher dye, while the right side contains the reporter dye.