Fig. 4

Relative gene expression results of (A) interleukin 8 (il8), (B) interleukin β 1 (ilβ1), and (C) nuclear factor kappa B subunit (nf-κB) in juvenile Nile tilapia (Oreochromis niloticus) raised under three distinct water temperatures (26, 28, and 30 ºC) and two feeding frequencies (1 and 2 meals/day) for each temperature over a two-month period. The fish cultured at a water temperature of 28 ºC and fed twice daily served as the control. Data are expressed as means ± SEM. *indicates P < 0.05, **indicates P < 0.01, and ***indicates P < 0.001