Fig. 5

Relative gene expression results of (A) complement C3 (c3), and (B) lysozyme (lyz) in juvenile Nile tilapia (Oreochromis niloticus) raised under three distinct water temperatures (26, 28, and 30 ºC) and two feeding frequencies (1 and 2 meals/day) for each temperature over a two-month period. The fish cultured at a water temperature of 28 ºC and fed twice daily served as the control. Data are expressed as means ± SEM. *indicates P < 0.05, **indicates P < 0.01, and ***indicates P < 0.001