Dietary tea tree (Melaleucae Aetheroleum) oil fortifies growth, biochemical, immune-antioxidant trait, gene function, tissue reaction, and Aeromonas sobria resistance in Nile tilapia (Oreochromis niloticus)

Table 2 Oligonucleotide primer sequences

Gene name	Primer sequences		NCBI accession no.	References
*β-actin*	F	TGACCTCACAGACTACCTCATG	XM_003455949.2	Abarike et al., [1]
*β-actin*	R	TGATGTCACGCACGATTTCC	XM_003455949.2	Abarike et al., [1]
*TNF-α*	F	CCAGAAGCACTAAAGGCGAAGA	NM_001279533.1	Standen et al., [44]
*TNF-α*	R	CCTTGGCTTTGCTGCTGATC	NM_001279533.1	Standen et al., [44]
*IL-1β*	F	TGGTGACTCTCCTGGTCTGA	XM_005457887.3	Standen et al., [44]
*IL-1β*	R	GCACAACTTTATCGGCTTCCA	XM_005457887.3	Standen et al., [44]
*TGF- β*	F	GTTTGAACTTCGGCGGTACTG	NM_001311325.1	Standen et al., [44]
*TGF- β*	R	TCCTGCTCATAGTCCCAGAGA	NM_001311325.1	Standen et al., [44]
*IL-10*	F	CTGCTAGATCAGTCCGTCGAA	XM_013269189.3	Standen et al., [44]
*IL-10*	R	GCAGAACCGTGTCCAGGTAA	XM_013269189.3	Standen et al., [44]
*Caspase-3*	F	GGCTCTTCGTCTGCTTCTGT	NM_001282894.1	Standen et al., [44]
*Caspase-3*	R	GGGAAATCGAGGCGGTATCT	NM_001282894.1	Standen et al., [44]
*IFN-γ*	F	GAAACTTCTGCAGGGATTGG	NM_001287402.1	Velázquez et al., [49]
*IFN-γ*	R	CTCTGGATCTTGATTTCGGG	NM_001287402.1	Velázquez et al., [49]
*BAX*	F	TACTTTGCATGCCGACTCGT	XM_019357746.2	Liu et al., [26]
*BAX*	R	CACCTTGCTCCCTGATCCAG	XM_019357746.2	Liu et al., [26]
*BCL2*	F	GACTGTACCAGCCGGACTTC	XM_003437902.5	Abdelaziz et al., [2]
*BCL2*	R	AAAGCAATAATCCGGCCCCA	XM_003437902.5	Abdelaziz et al., [2]

Beta actin (β-actin, house-keeping gene), interleukin 1beta (IL-1β), interleukin 10 (IL-10), and interferon gamma (IFN-γ), transforming growth factor beta (TGF- β), tumor necrosis factor alpha (TNF-α), BCL2 associated X protein (BAX), B cell lymphoma-2 (BCL2)

ISSN: 1746-6148