Rapid detection of Brucella cells using a gold nanoparticle-based aptasensor via a simple colorimetric method

BMC Veterinary Research

Table 1 Limit of detection of some methods for the detection of Brucella cells

Method	Recognition element	Sample matrix	Analyte	Analytical range	LOD	References
Electrochemical	Aptamer	Milk	B. melitensis	10² -10⁷ CFU/ml	10³ CFU/ml	[29]
Electrochemical	Antibody	Milk	B. melitensis	4 × 10⁴ & 4 × 10⁶ CFU/ml	1 × 10⁴ & 4 × 10⁵ CFU/ml	[30]
Optical (colorimetric)	DNA	Serum	B. melitensis	-	3.32 pg/ml	[7]
Optical (colorimetric)	DNA	Semen, Urine	B. abortus	10² × 10⁵ CFU/ml	10³ CFU/ml	[31]
Optical (colorimetric)	Antibody	Milk	B. abortus	1.5 × 10³-1.5 × 10⁸ CFU/ml	450 CFU/ml	[32]
Fluoro-immunosensing system	Antibody	-	B. melitensis	2.7–90 µg/l	2.7 µg/l	[33]
PCR assay	DNA	Milk	B. melitensis	1.7 × 10³ -1.7 × 10⁴ CFU/ml	1.7 × 10³ CFU/ml	[8]
Real-time PCR	DNA	Meat goat	B. melitensis	-	2 fg/ml	[34]
ELISA	Antibody	Serum	B. melitensis	10²-10⁸ Cell/ml	10³ Cell/ml	[35]
Optical (colorimetric)	Aptamer	Milk	B. melitensis	1.5 × 10¹-1.5 × 10⁸ CFU/ml	1.5 × 10¹ CFU/ml	This work

ISSN: 1746-6148