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Table 1 Composition and nutrient content of the experimental diet (dry matter, %)

From: Effects of supplementation with freeze-dried Clostridium butyricum powder after replacement of fishmeal with cottonseed protein concentrate on growth performance, immune response, and intestinal microbiota of Litopenaeus vannamei

Ingredient

CON

CB 0

CB 0.065

CB 0.26

CB 1.04

CB 4.16

Fish meal

25.00

15.00

15.00

15.00

15.00

15.00

Concentrated cottonseed protein

0.00

11.27

11.27

11.27

11.27

11.27

Shrimp shell meal

6.00

6.00

6.00

6.00

6.00

6.00

Dehulled soybean meal

10.00

10.00

10.00

10.00

10.00

10.00

Corn gluten meal

10.00

10.00

10.00

10.00

10.00

10.00

Peanut meal

10.00

10.00

10.00

10.00

10.00

10.00

Wheat flour

25.00

25.00

25.00

25.00

25.00

25.00

Fish oil

1.50

2.02

2.02

2.02

2.02

2.02

Soybean lecithin

2.50

2.50

2.50

2.50

2.50

2.50

Premix1)

1.00

1.00

1.00

1.00

1.00

1.00

Choline chloride

0.50

0.50

0.50

0.50

0.50

0.50

Vc

0.10

0.10

0.10

0.10

0.10

0.10

Ca(H2PO4)2

2.00

2.00

2.00

2.00

2.00

2.00

Attractant

0.10

0.10

0.10

0.10

0.10

0.10

Antioxidant

0.05

0.05

0.05

0.05

0.05

0.05

MCC

6.25

4.22

4.16

3.96

3.18

0.06

Methionine

0.00

0.07

0.07

0.07

0.07

0.07

Lysine

0.00

0.17

0.17

0.17

0.17

0.17

Freeze-dried Clostridium butyricum

0.00

0.00

0.065

0.26

1.04

4.16

Total

100

100

100

100

100

100

Crude protein

39.31

39.68

39.59

40.11

40.04

39.89

Crude lipid

7.34

6.18

5.94

6.14

6.08

6.16

Moisture

9.10

9.80

10.00

9.40

9.20

10.00

  1. 1) Premix offered by Qingdao Master Biotech Co., Ltd
  2. Vitamin and Mineral Premix (/Kg) includes following: Vitamin A, 500000IU; Vitamin D3, 100000IU; Vitamin E, 4000 mg; Vitamin K3, 1000 mg; Thiamine, 500 mg; Riboflavin, 1000 mg; Pyridoxine, 1000 mg; Cobalamin, 2 mg; Niacin, 4000 mg; Calcium pantothenate, 2000 mg; Folic acid, 100 mg; Vitamin C, 15,000 mg; Iron, 10,000 mg; Copper, 300 mg; Zinc, 5000 mg; Manganese, 1200 mg; Iodine, 80 mg; Selenium, 30 mg; Cobalt, 20 mg