Effects of supplementation with freeze-dried Clostridium butyricum powder after replacement of fishmeal with cottonseed protein concentrate on growth performance, immune response, and intestinal microbiota of Litopenaeus vannamei

Table 3 Primers used in this experiment for quantitative RT-PCR

Genes	Forward (5’-3’)	Reverse (5’-3’)	GenBank no.
β-actin	TGGACTTCGAGCAGGAGATG	GGAATGAGGGCTGGAACAGG	XM_027364954.1
Dorsal	TTGCGACCACCAGACAAGAG	GCAAGGTAACGACTAATCTTCTCTG	XM_027382195.1
Relish	CTACATTCTGCCCTTGACTCTGG	GGCTGGCAAGTCGTTCTCG	XM_027357254.1
PO	TACATGCACCAGCAAATTATCG	AGTTTGGGGAAGTAGCCGTC	XM_027379995.1
ALF	TTACTTCAATGGCAGGATGTGG	GTCCTCCGTGATGAGATTACTCTG	KJ000049
Pen-3	CACCCTTCGTGAGACCTTTG	AATATCCCTTTCCCACGTGAC	Y14926
Crus	CCACGAACCAGAGACACCTG	CGAGGCCAGCACACTTGTAG	AY486426
TOR	GCAGATCCTTGAGAAGACGC	CTGACAGCCGCATTGAGGTA	[26]
4E-BP	ATGTCTGCTTCGCCCGTCGCTCGCC	GGTTCTTGGGTGGGCTCTT	[26]
eIF4E1α	TCCCTTTCCCTAACCCTCA	GTTTTGCTGTCTCGCTTCC	[26]
IGF1	GTGGGCAGGGACCAAATC	TCAGTTACCACCAGCGATT	[41]
IGF2	CTCTGTACAGTCAGCCCAGC	CACACCCAGTCAGTCCCAAG	[41]

PO Phenoloxidase, ALF Anti-lipopolysaccharide factor, Crus Crustins, Pen-3 Penaeidin-3a, TOR Target of Rapamycin, 4E-BP eukaryotic translation initiation factor 4E-binding protein, eIF4E1α eukaryotic translation initiation factor 4E type 1-Alpha, IGF1 Insulin-like growth factor 1, IGF2 Insulin-like growth factor 2

ISSN: 1746-6148