Shaken, not stirred: magnetic bead DNA extraction as a rapid and effective method for the scaling up of bovine tuberculosis diagnosis

Table 2 Agreement between the VM qPCR and the true status of infection on samples extracted using the column-based approach

		True status			Positive Percent Agreement (95% CI)	Negative Percent Agreement (95% CI)	Kappa (95% CI)
		Positive	Negative	Total	Positive Percent Agreement (95% CI)	Negative Percent Agreement (95% CI)	Kappa (95% CI)
VM qPCR	Positive	70	1*	72	95.89% (88.46–99.14%)	98.61% (92.50–99.96%)	0.945 (0.892–0.998)
	Negative	3**	71	74
	Total	73	72	145

* This sample showed amplification for the QF qPCR with a Ct value above its diagnostic threshold (Ct > 38.0).
** Two samples were culture positive, whereas one was QF qPCR positive and IS1081 positive.

ISSN: 1746-6148