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Table 1 PCR amplification cycling conditions

From: Changes in the intestinal microbiota of broiler chicken induced by dietary supplementation of the diatomite-bentonite mixture

Cycle step

Temperature

Time

Number of cycles

Initial denaturation

95 °C

1 min

1

Denaturation

95 °C

20 s

30

Annealing

55 °C

30 s

Extension

65 °C

2 min

Final Extension

65 °C

5 min

1

Hold

4 °C

∞

 
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BMC Veterinary Research

ISSN: 1746-6148

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