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Table 3 Complete blood count (CBC) of Chelon ramada fed a basal diet supplemented with varing levels of rosemary oil and/or Zymogen Forte during a 75-day rearing trial in brackish groundwater

From: Dietary rosemary oil with/without zymogen forte improves water quality, growth hormones, immune-physiological response, stress resilience, and health status of Chelon ramada grown in groundwater

CBC

Treatments

P-Value

T1

T2

T3

T4

T5

RO

ZF

RO*ZF

Hb (g/100 ml)

10.15 ± 0.09c

11.35 ± 0.20b

11.60 ± 0.06ab

11.50 ± 0.12b

11.90 ± 0.06a

0.106

0.030

0.560

RBCs (×106/µL)

3.24 ± 0.02c

3.49 ± 0.02b

3.60 ± 0.01a

3.45 ± 0.03b

3.59 ± 0.03a

0.358

0.001

0.602

Hematocrit(HCT%)

29.21 ± 0.13c

32.03 ± 0.23b

33.49 ± 0.06a

32.05 ± 0.23b

32.91 ± 0.30a

0.248

0.001

0.215

MCV (µm3/cell)

90.15 ± 0.09c

91.90 ± 0.12b

93.15 ± 0.20a

92.9 ± 0.12a

91.80 ± 0.17b

0.295

0.644

0.000

MCH (pg/cell)

28.40 ± 0.23b

31.15 ± 0.20a

31.65 ± 0.20a

31.40 ± 0.69a

31.80 ± 0.12a

0.612

0.269

0.898

MCHC (%)

31.50 ± 0.29b

33.90 ± 0.18a

33.98 ± 0.14a

33.80 ± 0.70a

34.64 ± 0.19a

0.279

0.261

0.348

WBCs (×103/µL)

81.85 ± 0.14d

84.85 ± 0.66d

97.00 ± 0.92b

89.00 ± 1.15c

105.40 ± 2.08a

0.001

0.000

0.146

Lymphocyte (%)

68.00 ± 0.15c

79.00 ± 0.58b

86.50 ± 0.87a

86.50 ± 0.87a

77.50 ± 0.87b

0.001

0.001

0.000

Neutrophils (%)

14.0 ± 0.58b

27.5 ± 1.73a

8.50 ± 0.87c

8.0 ± 0.58c

15.5 ± 2.60b

0.007

0.010

0.000

Monocyte (%)

4.5 ± 0.29

3.5 ± 0.29

3.5 ± 0.29

3.5 ± 0.29

5.0 ± 1.15

0.267

0.267

0.267

Eosinophil (%)

2.5 ± 0.29

1.5 ± 0.29

1.5 ± 0.29

2.0 ± 0.00

2.0 ± 0.58

0.195

1.000

1.000

  1. Data are presented as mean ± SEM of 3 replicates, with different letters in the same row presenting significant differences (p < 0.05). (T1) = fish fed BD without RO or ZF (control); (T2) = fish fed the BD containing 0.5 g of RO kg− 1; (T3) = fish fed BD containing 0.5 g of RO and 1 g of ZF kg− 1; (T4) = fish fed BD containing 1.0 g of RO kg− 1; and (T5) = fish fed BD containing 1 g of RO and 1 g of ZF kg− 1; BD = basal diet, RO = rosemary oil; ZF = Zymogen Forte, RO*ZF = Interaction