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Table 5 Histomorphometric changes (goblet cell count, villi length and width, crypts of lieberkühn length and muscle coat thickness) in the midgut of Chelon ramada fed a basal diet containing different levels of rosemary oil and/or Zymogen Forte for a 75-day rearing trial in brackish groundwater

From: Dietary rosemary oil with/without zymogen forte improves water quality, growth hormones, immune-physiological response, stress resilience, and health status of Chelon ramada grown in groundwater

Parameter*

Treatments

P-Value

T1

T2

T3

T4

T5

RO

ZF

RO*ZF

Villi length, µm

485.6 ± 8.37c

435.0 ± 3.61c

510.4 ± 4.79c

609.6 ± 10.70b

815.6 ± 57.03a

0.000

0.001

0.055

Villi width, µm

123.58 ± 2.86b

92.28 ± 2.12d

105.86 ± 1.42c

115.85 ± 3.08bc

176.11 ± 6.16a

0.000

0.000

0.000

C. length, µm

185.05 ± 3.68a

119.88 ± 2.5c

69.41 ± 1.50e

83.85 ± 3.13d

171.92 ± 2.35b

0.000

0.000

0.000

M.C. Thick, µm

81.78 ± 2.52a

24.01 ± 1.23d

45.35 ± 1.47c

47.60 ± 1.91c

70.75 ± 1.45b

0.000

0.000

0.571

GCC

22.67 ± 1.76b

26.0 ± 2.08ab

31.33 ± 2.60a

16.0 ± 1.15c

22.67 ± 0.88b

0.001

0.011

0.723

  1. Data are presented as mean ± SEM of 3 replicates, with different letters in the same row presenting significant differences (p < 0.05). (T1) = fish fed BD without RO or ZF “control”; (T2) = fish fed the BD containing 0.5 g of RO kg− 1; (T3) = fish fed BD containing 0.5 g of RO and 1 g of ZF kg− 1; (T4) = fish fed BD containing 1.0 g of RO kg− 1; and (T5) = fish fed BD containing 1 g of RO and 1 g of ZF kg− 1; BD = basal diet, RO = rosemary oil; ZF = Zymogen Forte, RO*ZF = Interaction; C. length = crypts of Lieberkühn length; M.C. Thick = muscle coat thickness; GCC = Goblet cell count