Fig. 1

Scheme for the reverse genetics (RG) system of AKAVs. For construction of the transcription plasmid, full-length cDNA fragments of the L, M, and S segments were cloned into the TVT7R vector, containing T7 promoter and HDV sequence. To construct the helper plasmids, ORFs of the RdRp and N were cloned into the pTM1 vector. Three transcription plasmids and two helper plasmids were co-transfected into BHK/T7-9 cells. The supernatants of BHK/T7-9 cells were passaged in Vero cells, and the rAKAV-7, rAKAV-7(S-K0505), and rAKAV-7ΔNSs viruses were rescued