Table 6 Primers and PCR conditions for qPCR analysis
From: Screening for transcriptomic associations with Swine Inflammation and Necrosis Syndrome
Genes | Forward-Primer | Reverse-Primer | Length (bp) | Annealing temperature (°C) | GC-content forward/ reverse (%) |
|---|---|---|---|---|---|
GYPA | TTTTTGCAGTGATGGCTGGC | GGCACAGGCAAAGGTTTCTTT | 88 | 60 | 50/47 |
VEGFA | CTTGCCTTGCTGCTCTACCT | TCCATGAACTTCACCACTTCGT | 98 | 60 | 55/45 |
CCL2 | TGCCCAGCCAGATGCAATTA | CCGCTGCATCGAGATCTTCT | 76 | 60 | 50/55 |
CRP | CTGTCTATGCTGGTGGGACC | GCTTGACATACACCTCGCCA | 87 | 60 | 60/55 |
IGSF5 | CACAACTTCTCTGCCTCCGA | GCGGTGATCCAGTCCCTG | 88 | 60 | 55/66 |
TMEM52B | TTCATCCAGCTTCCTTGGGC | CAGGCCACACAGCAGAAGTA | 135 | 60 | 55/55 |
S100A12 | GCCCAACACCCTCAAGAACA | CAGCACATCAGTCACCAGGA | 124 | 60 | 55/55 |
GAPDH | CACCATCTTCCAGGAGCGAG | GCCTTCTCCATGGTCGTGAA | 102 | 60 | 60/55 |