Table 7 Thermal cycling conditions: fast cycling (for Applied Biosystems QuantStudio instruments and primer tm ≥ 60 °C)
From: Screening for transcriptomic associations with Swine Inflammation and Necrosis Syndrome
Step | Temperature (°C) | Time (in sec) | Cycles |
|---|---|---|---|
UDG activation | 50 | 120 | Hold |
Polymerase activation | 95 | 120 | Hold |
Denaturation | 95 | 1 | 40 |
Annealing/extension | 60 | 30 | |
Melt curve stage | Â | Â | Ramp rate |
Step 1 | 95 | 15 | 1.6 °C/sec |
Step 2 | 60 | 60 | 1.6 °C/sec |
Step 3 | 95 | 15 | 0.15 °C/sec |