Table 1 Primers used in real-time quantitative PCR
Target gene | Primer sequence | Reference |
|---|---|---|
Ī²-actin | F-5'-CATCAGGGAGTCATGGTCGGCA-3ā R-5'GCATACAGGTCTTTACGGATGTCC-3' | Gene bank JQ086312 |
IL-17 | F-5ā-ATGGGAAGGTGATACGGC-3ā R-5ā GATGGGCACGGAGTTGA-3 | Gene bank AM773756 |
IL-22 | F-5'-GCACCTACACCTTGGCTGAA-3ā R-5'TGTAGCAGCGGTTGTTCTCC-3ā | GenBank: AJ617782.1 |
IFN-Ī³ | F-5'-GTGAAGAAGGTGAAAGATATCATGGA-3' R-5'-GCTTTGCGCTGGATTCTCA-3' | [38] [38] |
IL-1Ī² | F-5'-CAGCCTCAGCGAAGAGACCTT-3' R-5'-CACTGTGGTGTGCTCAGAATCC-3' | |
IL-4 | F-5'-AATGACATCCAGGGAGAGGTTTC-3' R-5'-AGGCTTTGCATAAGAGCTCAGTTT-3' | [39] |
TLR-4 | F-5'-TGTGCTGGAAAAAGCTGCAC-3' R-5'-AGCTTTGTCTCACGGGTCAC-3' | Gene bank, KP410249.1 |
MUC-2 | F: 5'-CAGCACCAACTTCTCAGTTCC-3' R:5'-TCTGCAGCCACACATTCTTT-3' | [40] [40] |
IgA | F: 5'-ACCACGGCTCTGACTGTACC-3' R: 5'-CGATGGTCTCCTTCACATCA-3' |