Table 5 Primer sequences for RT-qPCR
Gene | Forward Primer | Reverse Primer | Size/bp | NCBI Reference Sequence |
|---|---|---|---|---|
β-actin | GAGAAATTGTGCGTGACATCA | CCTGAACCTCTCATTGCCA | 152 | NM_205518.2 |
Claudin-1 | CATACTCCTGGGTCTGGTTGGT | GACAGCCATCCGCATCTTCT | 100 | NM_001013611.2 |
Occludin | ACGGCAGCACCTACCTCAA | GGGCGAAGAAGCAGATGAG | 123 | NM_205128.1 |
ZO-1 | CTTCAGGTGTTTCTCTTCCTCCTC | CTGTGGTTTCATGGCTGGATC | 131 | XM_046925214.1 |
IFN-γ | AGCTGACGGTGGACCTATTATT | GGCTTTGCGCTGGATTC | 259 | NM_205149.2 |
IL-8 | CCAAGCACACCTCTCTTCCA | GCAAGGTAGGACGCTGGTAA | 176 | NM_205498.2 |
IL-10 | CGGGAGCTGAGGGTGAA | GTGAAGAAGCGGTGACAGC | 272 | NM_001004414.4 |
TGF-β4 | CGGGACGGATGAGAAGAAC | CGGCCCACGTAGTAAATGAT | 258 | NM_001318456.1 |