Fig. 3

CAD enhanced gut barrier integrity in Mccp-induced pneumonia mice (a) Claudin-1、(b) Occludin and (c) ZO-1 protein levels in colon tissue samples were determined using Immunohistochemistry. Scale bar, 100 μm. d Western blot technique was used to detect Claudin-1、 Occludin and ZO-1 protein levels in colon tissue samples. e Relative Claudin-1 protein abundance. f Relative Occludin protein abundance. g Relative ZO-1 protein abundance. *p < 0.05, **p < 0.01, ***p < 0.001, ns p > 0.05