BMC Veterinary Research

Table 2 qPCR amplification parameters

From: Effects of Qiamagu (Brassica rapa L.) polysaccharide on growth performance, immunity and gut health of yellow-feathered broilers

Step	Temperature	Time
Step 1. Predegeneration	95℃	30 S
Step 2. Circular reaction	95℃	10Sx40cycles
Step 2. Circular reaction	60℃	30Sx40cycles
Step 3. Solubility curve	95℃	15 S
	60℃	60 S
	60℃	60 S

Back to article page

ISSN: 1746-6148

Contact us

General enquiries: journalsubmissions@springernature.com