Table 3 Primers sequence of target gene and reference gene
Gene name | Primer sequence(5, ~ 3, ) |
|---|---|
TNF-α | F: GGCAATGAACCCTCCCCAGTA R: GGTTACAGGAAGGGCAACTCATC |
IL-1β | F: CAGCCTCAGCGAAGAGACCTT R: ACTGTGGTGTGCTCAGAATCC |
IL-2 | F: TGCTTTTAACCGTCTTTG R: GATGCTCCATAAGCTGTAGT |
Occludin | F: ACGGCAGCACCTACCTCAA R: GGGCGAAGAAGGAGATGAG |
MUC2 | F: TTCATGATGCCTGCTCTTGTG R: CCTGAGCCTTGGTACATTCTTGT |
ZO-1 | F: GGGATGTTTATTTGGGCGGC R: TCACCGTGTGTTGTTCCCAT |
β-Actin | F: GAGAAATTGTGCGTGACATCA R: CCTGAACCTCTCATTGCCA |