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Fig. 1 | BMC Veterinary Research

Fig. 1

From: Isolation and characterization of bovine coronavirus variants with mutations in the hemagglutinin-esterase gene in dairy calves in China

Fig. 1

Isolation and identification of BCoV. (a) HRT-18 cell infected with BCoV showing cytopathic effects (CPE) at 72 h, such as shrinkage, rounding, and nonadherence. (b) IFA of BCoV-infected HRT-18 cells using the anti-N antibody. (C) TEM images of BCoV. (d) The viral protein N was detected with western blotting using an anti-N Polyclonal antibody at 6, 12, 18, and 24 h post-infection. Full-length blots are presented in additional file 9. The black box is the cropped area

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