Fig. 1

Isolation and identification of BCoV. (a) HRT-18 cell infected with BCoV showing cytopathic effects (CPE) at 72Â h, such as shrinkage, rounding, and nonadherence. (b) IFA of BCoV-infected HRT-18 cells using the anti-N antibody. (C) TEM images of BCoV. (d) The viral protein N was detected with western blotting using an anti-N Polyclonal antibody at 6, 12, 18, and 24Â h post-infection. Full-length blots are presented in additional file 9. The black box is the cropped area