Fig. 2

Maximum-likelihood analysis in combination with 1,000 bootstrap replicates was used to derive a phylogenetic tree based on the complete genomes (a), ORF1a/b genes (b), S (c), and HE (d) protein sequences. The sequences were aligned and clustered by Clustal W in MEGA 7.0 software. Circles represent the BCoV strains from this study and the HE-insertion variants were marked with a green triangle and the HE-deletion variants were marked with a blue triangle