Fig. 1
From: Microencapsulated rrBNGF as an alternative ovulation induction method in rabbits
Effects of rrBNGF treatments on PC12 cell cultures. Control (RPMI complete medium), Micro-empty (empty chitosan microcapsules), Micro-NGF (50 ng/mL rrBNGF in chitosan microcapsules) and Micro-empty + NGF (50 ng/mL free rrBNGF + empty chitosan microcapsules) on days 3, 5 and 7 after medium supplementation. A Percentage of rounded cells, B fusiform cells and C star-like cells in relation to the total cell number. D Percentage of cells showing 1 neurite and E, more than 1 neurite in relation to the total cell number. F Percentage of cells displaying neurites shorter than 1 cell diameter and G longer than 1 cell diameter neurites in relation to the total cell number. H Percentage of cells with neurites in relation to the total cell number. I Percentage of cell viability in supplemented cells in relation to control cells. Data are shown as mean ± SD at days 3, 5 and 7 after medium supplementation. Statistically significant differences among days in each treatment group are indicated as *p < 0.05, **p < 0.01, ***p < 0.001