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Fig. 5 | BMC Veterinary Research

Fig. 5

From: Therapeutic effect of long-interval repeated subcutaneous administration of canine amniotic membrane-derived mesenchymal stem cells in atopic dermatitis mouse model

Fig. 5

Expression profiles of CD markers in T cells determined by flow cytometry analysis after stimulation of canine PBMCs with conA. (A) Representative FACs image of CTL, conA, conA + MSCs and ConA + Pime. PBMCs were treated with concanavalin A (conA; 5 μm/ml), conA combined with MSCs (1 × 105cells), conA combined with pimecrolimus (Pime; 100 ng/ml) until Day 8. The markers used were CCR7 (CD197) and CD45ra. Three independent analyses were conducted. (B) Representative FACs image of CTL, conA, conA + MSCs and ConA + Pime. PBMCs were treated with concanavalin A (conA; 5 μm/ml), conA combined with MSCs (1 × 105cells), conA combined with pimecrolimus (Pime; 100 ng/ml) until Day 8. The markers used were CD62L and CD45ra. Three independent analyses were conducted. (C) Quantification of CCR7-negative and CD45ra-positive cells through flow cytometry analysis in triplicate. mean ± SD; ** p < 0.01, *** p < 0.001. (D) Quantification of CD62L-negative and CD45ra-positive cells through flow cytometry analysis in triplicate. mean ± SD; * p < 0.05, ** p < 0.01, *** p < 0.001

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BMC Veterinary Research

ISSN: 1746-6148

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