Table 1 Primers used in this study

5’AOX-F

GACTGGTTCCAATTGACAAGC

To confirm the CaIFNα integrated into the genome

3’AOX-R

GCAAATGGCATTCTGACATCC

T7-F

TAATACGACTCACTATAGGG

To confirm the CaIFNα cloned into pET28a

T7-R

TGCTAGTTATTGCTCAGCGG

EGFP-F

AAAGCCACCATGGTGAGCAAGGGCGAGGAG

To amplify the EGFP gene containing SacI and HindIII restriction sites

EGFP-R

ATACAGTTCTAGATTACTTGTACAGCTCGTCCATGCC

ISRE-F

GACGGCCAGTGAATTCGAGCTC

To amplify the interferon-stimulated response element containing SacI and HindIII

ISRE-R

CAGCTATGACCATGATTACGCCAAGCTT

pGL3-EGFP-F

GAGCTCGGTACCTATCGATAGAGAAATGTTCT

To amplify pGL-EGFP vector containing SacI and HindIII

pGL3-EGFP-R

AAGCTTGGCATTCCGGTACTGTTGGTAA

CaIFNα1-F

TCTAGCATGCATATGATGTGTCACCTCCCAG

To amplify the CaIFN gene containing NdeI and HindIII restriction sites

CaIFNα1-R

TGCAGTTTAAAGCTTTTATTTACGACGACGGAT

P2-F

AGAAAAGAGAGGCTGAAGCTGAATTCATGTGT

To verify that the CaIFN gene has been connected to pPICZαA

P2-R

TCGACGGCGCTATTCAGATCCTCTTCTGAGATGAGTTT

CaIFNα2-F

TCACGCCGAATTCATGTGTCACTTGCCAGACA

To amplify the CaIFN gene containing EcoRI and XbaI restriction sites

CaIFNα2-R

TCTAGATTAATGATGATGATGATGATGAACTTTCTTCTTCTGCGTAGA

RVprimer4-F

CTAGCAAAATAGGCTGTCCC

To verify the construction results of plasmid pGL3-EGFP-ISRE.

RVprimer4-R

GACGATAGTCATGCCCCGCG