Table 2 Plasmids used in this study
From: Identification of interferon-stimulated response elements (ISREs) in canines
Plasmids | Description | Source/reference |
|---|---|---|
pET-28a(+) | Cloning vector, KanR | Millipore |
pPICZαA | Cloning vector, ZeoR | Thermo Fisher Scientific |
pET-28a(+)-CaIFNα | Interferon-alpha gene cloned in pET-28a(+), KanR | This study |
pPICZαA-CaIFNα | Interferon-alpha gene cloned in pPICZαA, ZeoR | This study |
pUC19 | Cloning vector, AmpR | This study |
pUC19-ISRE1 | Interferon-stimulated response element1 gene cloned in pUC19, AmpR | This study |
pUC19-ISRE2 | Interferon-stimulated response element2 gene cloned in pUC19, AmpR | This study |
pUC19-ISRE3 | Interferon-stimulated response element3 gene cloned in pUC19, AmpR | This study |
pUC19-ISRE4 | Interferon-stimulated response element4 gene cloned in pUC19, AmpR | This study |
pUC19-ISRE5 | Interferon-stimulated response element5 gene cloned in pUC19, AmpR | This study |
pUC19-ISRE6 | Interferon-stimulated response element6 gene cloned in pUC19, AmpR | This study |
pUC19-ISRE7 | Interferon-stimulated response element7 gene cloned in pUC19, AmpR | This study |
pUC19-ISRE8 | Interferon-stimulated response element8 gene cloned in pUC19, AmpR | This study |
pUC19-ISRE9 | Interferon-stimulated response element9 gene cloned in pUC19, AmpR | This study |
pUC19-ISRE10 | Interferon-stimulated response element10 gene cloned in pUC19, AmpR | This study |
pGL3 basic | Expression vector, AmpR | Promega Corporation |
pEGFP-C1 | Enhanced Green Fluorescent Protein gene expression vector, KanR | Clontech |
pGL3-EGFP | Enhanced Green Fluorescent Protein gene was cloned into pGL3 basic with NcoI and XbaI, AmpR | This study |
pGL3-ISRE1-EGFP | Interferon-stimulated response element1 gene cloned in pGL3-EGFP with KpnI and NcoI, AmpR | This study |
pGL3-ISRE2-EGFP | Interferon-stimulated response element2 gene cloned in pGL3-EGFP with KpnI and NcoI, AmpR | This study |
pGL3-ISRE3-EGFP | Interferon-stimulated response element3 gene cloned in pGL3-EGFP with KpnI and NcoI, AmpR | This study |
pGL3-ISRE4-EGFP | Interferon-stimulated response element4 gene cloned in pGL3-EGFP with KpnI and NcoI, AmpR | This study |
pGL3-ISRE5-EGFP | Interferon-stimulated response element5 gene cloned in pGL3-EGFP with KpnI and NcoI, AmpR | This study |
pGL3-ISRE6-EGFP | Interferon-stimulated response element6 gene cloned in pGL3-EGFP with KpnI and NcoI, AmpR | This study |
pGL3-ISRE7-EGFP | Interferon-stimulated response element7 gene cloned in pGL3-EGFP with KpnI and NcoI, AmpR | This study |
pGL3-ISRE8-EGFP | Interferon-stimulated response element8 gene cloned in pGL3-EGFP with KpnI and NcoI, AmpR | This study |
pGL3-ISRE9-EGFP | Interferon-stimulated response element9 gene cloned in pGL3-EGFP with KpnI and NcoI, AmpR | This study |
pGL3-ISRE10-EGFP | Interferon-stimulated response element10 gene cloned in pGL3-EGFP with KpnI and NcoI, AmpR | This study |